Effects of CXCR2 and CXCR4 on human cervical carcinoma HeLa cells

Objective To investigate the effects of CXC chemokine receptor-2(CXCR2) and CXC chemokine receptor-4(CXCR4) on migration,proliferation and secretion of vascular endothelial growth factor(VEGF) of HeLa cells.Methods HeLa cell migration was detected with Transwell method.Effects of CXCR2 and CXCR4 on the proliferation of the HeLa cells was detected with 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) assay.The expression of VEGF of the HeLa cells was detected with enzyme-linked immunosorbent assay(ELISA).Results After the knockout of CXCR2 and CXCR4,the mobility of the HeLa cells were 2.1±0.8 and 2.6±0.9,and the rate was 3.6±1.1 for the cells without the knockout,with statistically significant differences(P<0.05 for all).After the activation of CXCR2 and CXCR4,the proliferation rates of the HeLa cells were 86.1±15.3% and 83.9±15.9%,significantly higher than that of the cells without the activation(66.7±8.7%)(P<0.05 for all).In addition,the VEGF expressions of the HeLa cells with the activation of CXCR2 and CXCR4 were 6.84±0.19 pg/L and 6.59±0.14 pg/L and were significantly higher than that of the cells without the activation(0.74±0.03 pg/L)(P<0.05 for all).Conclusion High expressions of CXCR2 and CXCR4 in HeLa cells play an important role in the induction of migration,proliferation and secretion of VEGF of HeLa cells.