Profile of microRNA expression in esophageal cancer TE-1 cells

Objective To examine the profile of microRNAs(miRNAs)expression in esophageal cancer TE-1cells and to provide references for early diagnosis of esophageal cancer.Methods The esophageal cancer cells TE-1 and normal human esophageal cells HET-1A were cultured and their total RNA were isolated.Then,the cDNA was synthesized by reverse transcription and labeled with Cy3 fluorescence as probes,hybridized with microRNA gene chip,and scanned by laser scanner.The acquired image was analyzed for differentially expressed miRNAs.The real time reverse transcription-PCR(RT-PCR)was used for the determination of both five most up-and down-regulated miRNAs.Results A total of 676 miRNAs were screened from the TE-1 line cells,among which 113 miRNAs were statistically different from those of HET-1A cells(all P<0.05),including 76 up-regulated and 37 down-regulated miRNAs.The detection results of real time RT-PCR for 10 more differentially expressed miRNAs were consistent with those of gene chip.Conclusion The miRNAs differentially expressed between the esophageal cancer cells lines TE-1 and normal human esophageal cells HET-1A were obtained by miRNAs microarray.The study on these differentially expressed miRNAs could provide a good base for the study on development mechanism of esophageal cancer.