Establishment of consensus sequences of Pre-S, S, Pre-C/BCP gene of hepatitis B virus prevaling in areas with high hepatocellular carcinoma incidence in Fusui county of Guangxi

Objective To establish consensus sequences of Pre-S, S, and Pre-C/BCP gene of hepatitis B virus(HBV) isolated in Fusui county of Guangxi Zhuang Autonomous Region(Guangxi).Methods Genes of Pre-S, S, and Pre-C/BCP of HBV isolated in 89 asymptomatic carrier of HBV were sequenced and the genotypes of the genes were determined with homology analysis.Consensus sequences of HBV prevailing in Fusui county were established base on different genotypes.Results Compared with the genotype sequences of HBV(CDQ448627, EU439006) isolated in other parts of China recorded in GenBank, the consensus sequences of Fusui county are of great difference, with a maximaum variation of 2.07%.The difference in the consensus sequences between different genotypes of HBV isolated in Fusui county was greater than the difference between the HBV isolated in different areas, with a minimum variation of 2.87% and a maximum of 9.65%.Conclusion The consensus sequences of Pre-S, S, and Pre-C/BCP gene of HBV isolated in Fusui county were primarily established and the study lays a foundation for researches on genetic mutations of HBV in Fusui county.