Pathogen detection for viral diarrhea among infants in Lulong county of Hebei province, 2010-2014

Objective To study pathogens of viral diarrhea among infants in Lulong county of Hebei province. Methods Medical records of all diarrhea patients aged less than 5 years and hospitalized in Lulong County Hospital during 2010 and 2014 were collected and stool specimens of the inpatients were collected for viral pathogen detection with enzyme-linked immunosorbent assay (ELISA) and revere-transcription polymerase chain reaction (RT-PCR). Results For the 1 889 stool specimens detected, the overall virus positive rate was 72.1 %(1 356)and the positive rate of human rotavirus(HRV), human caliciviruses(HuCV), adenovirus(AdeV), and astrovirus(AstV)were 45.0%(846), 18.3%(344), 6.3%(119), and 2.5%(47), respectively.More positive specimens were detected during December to January of the next year for HRV, January to March in a year for HuCV, November to April of the next year for AdeV, and February to March in a year for AstV.More than or near one third of the HRV, HuCV, and AstV positive cases were aged 12-17 months, with the proportion of 38.1%, 32.6%, and 38.2%;while more than one fifth (21.8%)of AdeV positive cases were aged 9-11 months.The dominant serotype of HRV strains identified was G9 (57.1%), with a dominant genotype of P8;the dominant serotype of the HuCV strains was GⅡ. Conclusion HRV is a main pathogen of diarrhea among infants in Lulong county of Hebei province from 2010 to 2014.