Effect of ALDH1A1 on biological behavior of gastric cancer cells through Wnt signaling pathway

Objective To explore the effect of aldehyde dehydrogenases 1A1 (ALDH1A1) on biological behavior of gastric cancer cell MKN-45 through wingless protein (Wnt) signaling pathway.Methods MKN-45 cells were transferred with the control and sh-RNA-ALDH1A1.The expression of ALDH1A1 in MK-45 cells was determined with reveres transcriptase-PCR (RT-PCR) and Western blot.The cell viability was detected with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.The cell migration and invasion were detected with transwell method.The expressions of matrix metalloproteinase 2 (MMP2),matrix metalloproteinase 9 (MMP9),Wnt1,Wnt5a,and β-catenin were detected with Western blot.Results Compared with those of the control group,the ALDH1A1 transfected cells showed significantly down-regulated expressions of ALDH1A1 protein (0.89±0.09 vs. 0.09±0.01) and mRNA (0.48±0.05 vs. 0.08±0.01),decreased cell viability (0.73±0.07 vs. 0.40±0.04),inhibited cell migration (98.86±9.03 vs. 39.78±3.24) and invasion (90.52±9.14 vs. 42.53±4.25),and down-regulated expressions of MMP2 (0.49±0.04 vs. 0.10±0.01),MMP9 (0.95±0.09 vs. 0.10±0.01),Wnt1 (1.29±0.12 vs. 0.28±0.03),Wnt5a (0.52±0.04 vs. 0.12±0.01),and β-catenin (0.56±0.05 vs. 0.19±0.02) (P<0.01 for all).Conclusion ALDH1A1 silence could decrease cell viability and inhibit cell migration and invasion via regulation of Wnt signal pathway in MK-45 cells.