Effect of admixture of cholera toxoid B and insulin on spontaneous autoimmune diabetes

  Objective   To observe the diabetes prevention effects of oral administration of the admixtures of insulin and cholera toxin B subunit(CTB)on female non-obese diabetic mice, and to offer evidence for constructing diabetes administrative project in community.

  Methods   40 3-weeks-old female NOD mice were divided randomly into four groups.Oral administr ation persisted from 4-weeks-old to 12-weeks-old.The whole observation lasted to 26-weeks-old.Glycosuria and glycemia were measured from 12 weeks to 26 weeks.The cumulative incidence rate were compared to estimate the effect of oral administration.

  Results   Oral administration of CTB+insulin 100 μg, CTB+insulin 1 mg and insulin 1 mg to female NOD mice significantly suppressed incidence of diabetes than PBS group.The cumulative incidence rate of PBS, CTB+insulin 100 μg, CTB+insulin 1 mg and insulin 1 mg were 100%, 60%, 60%, 40%(P < 0.05).And two examination groups had no significant difference with the insulin 1 mg group.

  Conclusion   Oral administration of CTB+insulin 100 μg, CTB+insulin 1 mg to female NOD mice significantly can suppress incidence of diabetes and enhance the diabetes prevention effect than PBS group.