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WANG Wenjing, RUI Yongyu, CHEN Yao, . Study on polymorphism of 16s-23s rRNA ribosomal-spacerregion of Brucella in China[J]. Chinese Journal of Public Health, 2005, 21(10): 1172-1173.
Citation: WANG Wenjing, RUI Yongyu, CHEN Yao, . Study on polymorphism of 16s-23s rRNA ribosomal-spacerregion of Brucella in China[J]. Chinese Journal of Public Health, 2005, 21(10): 1172-1173.
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Study on polymorphism of 16s-23s rRNA ribosomal-spacerregion of Brucella in China

    Abstract
  •   Objective   To identify Brucella spp by 16 s-23 s rRNA Ribosoma-l Spacer-Region(ITS)and to estimate the significance of ITS as gene labelling in Brucella.
      Methods   Strain typing of 120 Brucella spp. in China by PCR-SSCP analysis and sequencing for ITS.
      Results   Four types(Ⅰ, Ⅱ, Ⅲ, Ⅳ)existed in all species which were examined. Sequence analysis of the ITS revealed a spacer of 765 bp with high(99.87%)homology and only 3 sites were different.
      Conclusion   ITS with high homology is of some importance to be marker in Brucella spp.
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