Toxic effect of Ascaris lumbricoides body fluid on intestinal epithelial cells

  Objective   To study the toxic effect of body fluid of Ascaris lumbricoides(ABF)on human intestinal epithelial cells (HCT-8 cells)and how the ABF concentration and inducing time could be related to the toxicity.

  Methods   Survival rates, morphology and function of HCT-8 cells were compared in different ABF concentrations(i.e., 30, 100, 400 μg/ml)and different time in vitro.

  Results   The observation from HE stain showed the most tox ic effect of HCT-8 cells at 800 μg/ml for 8 h. The toxicity of HCT-8 cels in each experiment group(25~800 μg/ml)was higher than that of matched control except 12.5% μg/ml.

  Conclusion   The toxicity induced in vitro by ABF implies a dose-and time-dependent pattern in some extension. Meanwhile, ABF induced the apoptosis of HCT8 cells.