Objective To explore the effect of garlic oil combining with resveratrol inducing apoptosis of human gastric carcinoma cell line MGC-803.
Methods To measure the inhibit effect of proliferation on garlic oil combining with resveratrol to gastric carcinoma cell by MTT. Apoptotic effect of gastric carcinoma cell by giemsa staining was observed. TUNEL assay, flow cytometry and confocal microscopy were used.
Results The inhabiting rate of 42.8%, 59.8% and 65.5% could be measured after being exposed to garlic oil combining with resveratrol at the dosage 25μg/ml for 6h, 12h and 24h. Obvious cell apoptosis could be observed by giemsa staining and TUNNEL staining after exposed to combining medicine at 25 μg/ml for 24h. Positive cells of Annexin Ⅴ in group of garlic oil combined with resveratrol were higher than those of total positive cells of both garlic oil group and resveratrol group. The reverse results could be observed about DNA content within cells of the combined medicine group and the single medicine group by confocal microscopy.
Conclusion The effect of apoptosis on garlic oil combined with resveratrol to MGC-803 cell line is obviously stronger than that of their single usage.
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