Study on determination of taurine and its stability in beer by fluorophotometry

  Objective   To determine the content of taurine in beer by fluorophotometry and its stability in beer.

  Methods   The normal beer was incorporated with taurine at five levels and kept under 4℃ and normal temperature respectively for use. Freshly incorporate beer was made six months later and was determined with the formerly incorporate beers all together. After pretreatment, the sample was separated and cleaned up with cation ex change column, then taurine was derived by o-phthalaldehyde and was monitored by fluorophotometry.

  Results   T he fluorophotometry for taurine analysis in beer showed the precision(relative standard deviation)was less than 2.4%, the recoveries were more than 95%and the detection limit was 0.023 mg/L; The differences of taurine content among all the samples were not statistically significant(P > 0.05).

  Conclusion   The method is rapid, simple, with a good reproducibility and is suitable of analysis of taurine in beer; the good stability of taurine in beer ensures the feasibility of taurine-contained hygienical beer.