Objective To establish a two-site antibo dyenzyme-linked immunosorbent assay(ELISA)for detecting food specimens artifically contaminated with employed as capture antibody.
Methods A monoclonal antibody to staphylococcus enterotox in B(SEB)2D1 was used as indicator antibody.The sensitivity of ELISA was improved by biotin Ostrepra bindins system.
Results Calibration curves for SEB were linear from 0.078-10.000 μg/L(r=0.987 6), and the mean co-efficient of variation(CV)was 4.99%.
Conclusion This method is simple, rapid with high sensitivity and stablity, which may be suitable for quantitative measurement of the SEB in both food specimens.
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