Synthesis of α-tocopherol succinate and study on its function form in inhibiting SGC-7901 cell growth

  Objective   To synthesize α-tocopherol succinate(α-TOS); to examine its effect on the growth of human gastric carcinoma cell(SGC-7901)and to explore its molecular function form.

  Methods   α-TOS was synthesized by reactingα-tocopherolα(-TOH)with succinic anhydrite in the presence of catalyst.Human gastric cancer SGC-7901 cell in vitro was used as the experimental cell, the inhibitory effect of α-TOS on the cell proliferation was investigated with 3-(4, 5-dimethyl-thizazol-2-yl)-2, 5-diphenyl tetrazolium bromide(MTT)method and its inducibility of cell apoptosis by DNA ladder.Identification of the molecular function form of α-TOS was accomplished by High Performance Liquid Chromatography(HPLC).

  Results   The analytic result of the infrared spectrometry and mass spectrometry indicated the synthesis of α-TOS.Compared with the control groupα, -TOS at 10 and 20μg/ml had significatly inhibitory effect on the proliferation of SGC-7901 cells, whose survival rates were 21.7% and 7.7%, respectively, after 6-day treatment.The typical DNA ladders were observed in SGC-7901 cells after 48 administration ofα-TOS at concentrations 10 and 20μg/ml.α-TOS was detected in the cells and culture medium by HPLC, but its hydrolyzed objectα-TOH was not found.

  Conclusion   The synthesizedα-TOS can inhibit the proliferation of SGC-7901 cells and induce the apoptosis of the cells in dose-dependent manner; α-TOS inhibit SGC-7901 cell growth by its intact form.