Case-control study on relationship of CYP1A1 and GSTM1 polymorphisms and susceptibility to esophageal squamous carcinoma

  Objective   To study the relationship of the genetic polymerphisms of CYP1A1 and GSTM1 and susceptibility to esophageal squamous cell carcinoma (ESCC) and the gene-gene and gene-environment interaction.

  Methods   A case control study which included 89 cases of esophageal cancer and 98 controls collected from the ChangZhi HePing Hospital and the ChangZhi HeJi hospital.All subjects were investigated with a uniform questionnaire.Genotyping of both CYP1A1 and GSTM1 were detected in blood samples of all subjects by PCR and PCR-RELP.Cobined genetic factors with environmental factors, the interaction of gene-gene and gene-environment was analyzed.

  Results   Only CYP1A1 Msp I mutation genotype showed significant difference between the two groups, with an OR_B of 1.93(95%CI=1.01~3.84), with an OR_C of 3.62(95%CI=1.61~8.14).There were on signoficant differences in the frequency distribution of three genotypes of CYP1A1 Ile-Val polymorphisms and GSTM null genotype between ESCC patients and controls.Combined effects of the CYP1A1 Msp I mutation genotype, and GSTM1 mull genotype indicated a notable interaction for increasing the regression genotype and GSTM1 mull genotype indicated a notable interaction for increasing the risk of ESCC, with an OR of 3.57(95%CI=1.36~9.41).No significant interaction was observed between the mutation genotype of CYP1A1 Ile-Val polymorphism and GSTM1 null genotype.Multiple Logistic regression models showed that CYP1A1 Msp I mutation genotype and family history of esophageal cancer(EC)were significant risk factors in genetic.The analytical results of combined effects showed that the effects of taking in fresh vegetable and fruit and eggs and CYP1A1 Msp I mutation genotype were resistant(s=0.26;s=0.48 respectively), The combined effects between family history of EC and CYP1A1 Msp I mutation genotype were synergistic(s=2.36).

  Conclusion   CYP1A1 Msp I polymorphisms was susceptible to ESCC.Combined effects of the CYP1A1 Msp I mutation genotype with family history of EC were synergistic.Subjects with either CYP1A1 Msp I mutation genotype or family history of EC were the higher risk population of ESCC, and more attentions should be paid to in the carcinoma control program.