Production and preliminary application of anti-staphylococcal enterotoxinB monoclonal antibodies

  Objective   To prepare Anti-staphylococcal EnterotoxinB(SEB)Moncolonal antibodies.

  Methods   Hybridomas secreting Anti-SEB Monoclonal Antibodies were developed by fusion of SP2/0 myeloma cells and splenocytes from BALB/C mice immunized with purified SEB.Six positive clones Anti-SEB Monoclonal Antibodies were obtained by repeated screening and clonings

  Results   6 strains belonged to Ig Glsubclass except two strains(IgG2-a)and their titers in culture supernatant and ascitic fluid by ELISA were between 1:256~1:512 and 1:12 800~1:25 600 respectively, It was revealed that 2 strains had very weak cross-reaction to SEC1, All Monoclonal Antibodies recognized a 28.5Kda protein which was defined as SEB by Western-blot analysis which indicated McAbs with high specificities were successfully established.

  Conclusion   McAbs against SEB were successfully obtained and could be used to detect SEBin hospital and test department.