Study on interaction between nitrophenols and DNA in hybridization

  Objective   To study the interaction between nitrophenols and deoxgribonucleic acid in hybridization.

  Methods   Ultraviolet-visble spectrophotometry, fluorescence spectrophotometry, cyclic voltammetry, hybridization and quantum chemistry were used to study the interaction between nitrophenols and deoxgribonucleic acid.

  Results   In pH 7.42 sodium phosphate buffer solution, the complex could be formed in hybridization, which decreased the deoxidized current of nitrophonals, and changed the peaks of absorption and decreased the deoxidized current of nitrophonals, and changed the peaks of absorption and fluorescence spectra.The theoretical calculation indicated that the nitrophenols closed with the base groups of DNA.

  Conclusion   Ⅱ-Ⅱ bonding was the major modes for the interaction between nitrophenols and DNA in hybridization.