Objective DNA damage in human lymphocyte cells of drug-users was monitored with comet assay.
Methods 123 drug-users were randomly selected as exposure group from a detoxification setting in Hubei and 79 not exposed to drug as control group.DNA damage in their lymphocyte cells was detected with comet assay.
Results Rate of DNA tail was 10.03% in exposure group, significantly higher than that in control group(1.26%), with very statistical significance, and tail length was(2.35±0.88)min exposure, significantly higher than that in control group(2.05±0.76)um.Rate of DNA tail was 1149% in exposure group with long length of drug-addicts, significantly higher than that with short length of drug-addicts(2.98%).Multivariate unconditional logistic regression analysis showed that possibility of DNA damag e was significantly higher in exposure group than that in control group(P < 0.05).
Conclusion Drug-addicts could cause certain damage to human lymphocyte cell DNA, the longer time of drug-addicts, the more serious damage of DNA.
DownLoad: