Effect of genotypes and haplotypes of tumor necrosis factor α and β on the susceptibility of Systemic Lupus Erythematosus

  Objective   To make sure whether there was any association between genotypes and haplotypes of tumor necrosis factor(TNF)α, β and SLE.

  Methods   PCR-RFLP method was used to type the genotype of the subjects.A case-control study was carried out including 99 SLE patients and 116 health controls from East China.

  Results   Compared with subjects with the genotype of TNFα1-TNFβ1, those with TNFα1-TNFβ2, TNFα2-TNFβ1 or TNFα2-TNFβ2 had higher risk of suffering from SLE(OR=2.31, 95.0%CI: 0.99-5.57;OR=2.60, 95.0%CI: 0.47-13.86;OR=9.53, 95.0%CI: 1.88-61.17).And there was a trend of increasing risk among different genotypes(χ²=10.16, P=0.001).The linkage disequi- librium was observed in subjects under study with TNFα and TNFβ(exact P=0.000).There were two types of susceptible haplotypes i.e.TNFα2-β1 and TNFα1-β2(P < 0.05).

  Conclusion   There were statistical associations between genotypes and haplotypes of tumor necrosis factor(TNF)α, β and SLE.