Molecular epidemiological investigation on HIV-1 epidemic strain

  Objective   To investigate the subtype of HIV-1 prevelent in Guangdong province.

  Methods   HIV-1 proviral DNA from buffy coat of 108 infectors in Guangdong was amplified by nested PCR.The C2-V3 regions of HIV-1 ENV gene were sequenced directly from the PCR product and were analyzed.

  Results   The 108 infectors were confirmed to be infected with five HIV-1 subtype or Circulating Recombinant Form(CRFs): AE(n=44), 072BC(n=40), 082BC(n=14), B'(n=9)and B(n=1).The AE group showed the highest homology to 01AE.TH.90.CM240 strain isolated in Thailand, and the genetic distances was(8.644±1.273)%.The 07-BC group showed the highest homology to CN.97.C54A strain, and the genetic distances was(2.163±1.313)%.The 08-BC group showed the highest homology to 97CNGX-9F strain, the genetic distances was(2.757±2.027)%.The B′group showed the highest homology to r142 strain isolated in Thailand, and the genetic distances was(5.87±2.232)%.THe B subtype showed highest homology to US.86.JRFL strain isolated in USA, and the genetic distance was 8.50%.The innergroups distance among AE, 07-BC, 08-BC, B'group were(7.302±4.639)%, (31388±11686)%, (3.728±1.971)%, (3.728±1.971)%, respectively.

  Conclusion   AE and 07-BC were main epidemic subtypes in Guangdong province.08-BC, B' and B were also circulating in Guangdong province.