Effects of 2, 5-hexanedione on cytoskeletal proteins in cerebrum of rats

  Objective   To investigate the alterations of cytoskeletal proteins in the cerebrum of 2, 5-hexanedione(HD) intoxicated rats.

  Methods   HD was administered by intraperitoneal injection to Wistar rats with daily dosing of 200 and 400 mg/kg for continuous 8 weeks(five times every week).Cerebrum tissues were homogenized and centrifuged at 28.000 g for 30 min to prepare the supernatant and pellet sample.The relative levels of light neurofilament(NF-L), midsized neurofilament(NF-M), heavy neurofilament(NF-H), β-actin, α-tubulin and β-tubulin were determined by SDS-PAGE and Western-Blot.

  Results   Excep that the contents of NF-L and NF-M couldn't be assayed for the reason of low concentration, HD intoxication resulted in a dose-dependent reduction of NF subunit proteins in the supernatant and pellet to 33%-73%.The contents of β-actin, α-tubulin and β-tubulin remained relatively unaffected in the pellet and the supernatant compared with the control group.

  Conclusion   HD induced alterations in cytoskeletal proteins in the cerebrum of rats, and the alterations were related to its peripheral and central neurotioxicity.