Study on oxidative DNA damage induced by PAHs from household pollution

  Objective   To study the kinds and concentration of PAHs released from household pollution-environmental tobacco smoke and heated oil smoke, and the effect of inducing oxidative DNA damage.

  Methods   Collected particles of heated oil smoke and environmental tobacco smoke were quantitatively detected by GC-MS-SIM.With the trachea instillation, 8-hydroxy-2'-deoxyguanosine, the biomarker of oxidative DNA damage from the rat lung tissue induced by the 10 kinds of PAHs', was detected by HPL C-EC.

  Results   PAHs widely existed in the condensed matters and the particle matters such as heated oil, the main and the side stream tobacco smoke.The mixture compounds of 10 kinds standard PAHs can induce oxidative DNA damage of the rat lung tissue to form 8-OHdG, and had significnat dose-response relationship.

  Conclusion   Genotoxic and carcinogenic effects of PAHs had a path of after metabolized to yield hydroxyl radicals(°OH)to attack DNA.