Organic distribution of dengue virus in infected mouse

  Objective   To analyse the mechanism of dengue virus infection by observing the organic distribution of virus in the infected mouse.

  Methods   Virus isolation and RT-PCR were used to detect the virus and viral RNA.The time-course detection of virus in the mouse blood plasma and liver, spleen, kidney, brain samples were observed.

  Results   The virus existed in the mouse blood plasma and liver, spleen, kidney, brain after mouse were inoculated with dengue virus(NGC strain, clinic strain).The virus duration in the brain was longest.Further more, the virus existence in the mouse infected with clinic strain dengue virus was longer than the group of NGC strain infection.

  Conclusion   The dengue virus was systemic distribution in the infected mouse.And the virulence of clinic strain dengue virus was harder than NGC strain.