Effect of lead on NOS1 expression in brain of developmental mouse

  Objective   To study lead effect of different concentrations on the expression of Nitric Oxide Synthase(NOS1) in mouse brain of developmental period and relation with learning memory function of mouse.

  Methods   To take out the brain tissue of mouse that exposed to differ ent concentration of lead in 5 time segments, such as, P1(postnatal 1d), P8, P15, P22, P30 etc.respectively dividid into groups after homogenate and then proceed to Westem blot experiment.

  Results   The expression of NOS1 in mouse brains of control group decreased gradually from P1 to P30;and in the group of low concentration of lead exposure(0.5 mmol/L), NOS1 increased in a few time segment; and in the groups of higher concentration of lead exposure(2.4-7.2 mmol/L), the expression of NOS1 increased in more time segments; but in the highest concentration groups (9.6-14.4 mmol/L), the NOS1 expression decreased gradually.

  Conclusion   The expression of NOS1 in lead-exposed young mouse brain were different to that in the groups without lead exposure, which indicated that NOS1 may have important relation with development of learning memory function.