Effect of soybean isoflavone on expression of bone BMP₂ and TGF-β₁ in ovariectomized rats

  Objective   To study the effect of soybean isoflavone and calcium on mineral and the expression of bone BMP₂, TGF-β₁ in ovariectomized rats.

  Methods   Twenty eight female SD rats were randomly divided into four groups: sham, ovariectomized, ovariectomized added calcium〔50 mg/(kg°d)〕, ovariectomized added soybean isoflavone〔100 mg/(kg°d)〕.The content of serum and urine calcium, sodium, potassium were deter mined after ten weeks.The Streptavidin-Peroxidase Histostain^TM-Plus Kits was used to detect bone BMP₂ and TGF-β₁ expression.

  Results   The calcium, sodium of serum and urine in ovariectomized group increased significantly.The bone mineral density, and potassium of serum and urine, bone BMP₂ and TGF-β₁ decreased significantly.The serum calcium and urine calcium, sodium in ovariectomized added soybean isoflavone group decreased significantly, but the bone mineral density and bone BMP₂ and TGF-β₁ expression increased significantly(P < 0.05 orP < 0.01).Only the TGF-β₁ expression in ovariectomized added calcium group had significantly increace than that of ovariectomized group(P < 0.05).

  Conclusion   The soybean isoflavone may adjust the mineral metabolism and prompt the bone BMP₂, TGF-β₁ expression to prevent the osteoporosis of ovariectomized rats.