Construction of an eukaryotic expression vector pcDNA3.1-glutathione-S-transferase M1 and sequence determination

  Objective   To construct an eukaryotic expression vector carrying human glutathione-S-transferase(GST)M1 gene and to determine partial sequence analysis.

  Methods   The GSTM1 cDNA was amplified and extracted from human lung total RNAs by RT-PCR approach and recombined with eukaryotic expression vector pcDNA311.The recombined plasmid pcDNA 3.1-hGSTM1 was verified using PCR, restriction analysis and sequencing determination.

  Results   Human GSTM1 gene was recombined correctly with pcDNA311, compared with genbank, in code 619 C→A, amino acid Pro→Thr, in code 519, C→G, in code 528, C→T, animo acids were not changed.GenBank accession was A Y532926.

  Conclusion   The eukaryotic expression vector pcDNA3.1-hGSTM1 was constructed for research detoxicitic mechanism in eukaryotic cell tranins and animals.