Effect of nickel sulfide on gene in 16HBE cells

  Objective   To detect the alterations of k-ras gene and p15 gene and analyse the genomic instability in the malignant process of 16 HBE cells induced by crystalline nickel sulfide (NiS).

  Methods   PCR-RFLP was used to detect the mutation of 12 code of k-ras gene and PCR-SSCP was used to examine the presence of alterations of k-ras gene exon 1 and p15 gene exon 2 in the malignant process of 16 HBE cells induced by crystalline nickel sulfide. Analysing the genomic instability by random amplified polymorohic DNA (RAPD).

  Results   Compared with the negative control cells, alterations of p15 gene and k-ras gene were not observed and genomic instability was showed in the malignant process of 16 HBE cells induced by crystalline nickel sulfide.

  Conclusion   Crystalline nickel sulfide could not induce alteration of p15 gene and k-ras gene, which indicated these four nickel compounds had not effect on the P15 gene exon2 and K-ras gene exonl, but they could induce genomic instability, furthermore, having special PCR bands, indicating genome became more instability in the malignant process.