Objective In order to detect the gene homologies of orientia tsutsugamushi (Ot) isolated from scrub typhus patients, chigger mites, and rodents of Shandong province in epidemic season.
Methods Nested-PCR connected with PCR/RFLP was used to identify the gene type of 12 strains Ot isolated from scrub typhus patients, the dominant chigger mite Leptotrombidium (L.) scutellare, and the dominant rodent Apodemus (A) agrarius in epidemic season of scrub typhus, 2 pools of homogenized L. scutellare, and 10 blood of scrub typhus, and compared with international reference strains Gilliam、Karp、Kato.
Results Using PCR/RFLP, 24 had the similar restriction maps with Japan Kawasaki strain, but they had not restriction site of Hha Ⅰ, and thus had some difference in gene sequence compared with Japan Kawasaki strain. Using Nested-PCR, 24 had the same products in the amplification of template Ot-DNA, thus they all belonged to Kawasaki strains, which was endemic in Japan.
Conclusion The gene types of Ot isolated from scrubty phuspatients, chigger mites, and rodents of Shandong province in epidemic season were identical, they all were similar to Japan Kawasaki strain, buthad some differences in gene sequence, and the transmission of Ot among patients, chigger mites, and rodents existed in Shandong province.
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