Protective effects of TFBP on human immortalized keratinocytes line HaCaT

  Objective   To investigate the damage of lead acetate and sodium arsenite on human immotalized keratinocytes line HaCaT and the protective effects of TFBP(total flzvonoids of broussonetia papyrifera)gotten from the leaves of broussoneria papyifera.

  Methods   TFBP were gotten by use of the absorption chromatograph column of polyamide. MTT reduction assay were performed and the levels of malondialdehyde (MDA)were determined in cultured HaCaT cells. Human immotlaized keratinocytes HaCaT cells were irradiated by lead acetate and sodium arsenite radiation at different dosages. The protective effects of TFBP also were evaluated.

  Results   With the different concentrations of 0.1-1.0 mmol/L lead acetate, the skin cells were markedly inhibited. Sodium arsenite at the lower concentrations of 0.5-1.0μmol/L induced mainly the degree of the proliferation of the HaCaT cells, while inhibiting cell growth over 5μmol/L.TFBP at the higher concentrations of 100 mg/L had certain protective effects from lead acetate or sodium arsenite, and the levels of MDA decreased, too.

  Conclusion   Lead acetate and sodium arsenite could cause significant damage to HaCaT cells under the conditions of this study, TFBP gotten from the leaves of broussoneria papyifera had certain protective effects on HaCaT epithelial cells from those damage.