Relationship between pharmaceutical metabolism enzyme and inhibitory effect of VES on B (a) P

  Objective   To study the role of the pharmaceutical metabolism enzyme during the inhibitory effect of VES against B (a) P in mice.

  Methods   The model of B (a) P-induced forestomach cancer in mice would be established firstly to check whether there was any difference between the two kinds of demonstrating ways. Then to select the best way to do the following experiment. The activities of EROD and GST in liver S-9 fraction were measured by spectrophoto fluorometer and GST kit, respectively.

  Results   VES both in oral and ip. can decrease the development of B (a) P-induced forestomach cancer in mice. While the activity of EROD, phase ⅳ pharmacological metabolism enzyme, in the VES group(39.1±3.05), were decreased significantly(P < 0.01), compared with the B (a) P group (57.9±3.16).

  Conclusion   VES can decrease the carcinogenesis of forestomach cancer in mice involving the depressing activities of EROD.