Objective To realize the transformation shape of influenza virus under the action of split, and the action about the virus substructure shape. To learn split way.
Methods To draw split influenza viruse specimen of A1, A3 and B subtypes at different time, and observe under electron microscope. The split solvent is Triton N-101.
Results The splitting of influenza virus can be divided into four stages. The stage includ M protein (Mp) membrane swell neuraminidase (NA) hemagglutinin (HA) lipid bilayer (LB) break away phase, Mprotein (Mp) membrane disrupt, nucleocapsid (NP) spill and Mprotein dissolved phase.
Conclusion The method of vibration makes split better.