Detection of purity and impurity of ebselen and security test

  Objective   To understand the purity and the impurity of the synthesized ebselen (Ebs) and the acute toxicity.

  Methods   The Ebs was tested with HPLC, the impurity was detected with chromatometry and the acute test was done by adultratlav aged one time and the 24-hour mortality rate was observed.

  Results   The purity of Ebs was 99.95%-99.99%, the amount of phenylamine was 0.000 17%-0.000 19%, the LD₅₀ of the rat lavaged by mouth one time was 2 355.05mg/kg.

  Conclusion   The quality of Ebs was reliable, the security was high, and it could be used for exploitation and study.