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SONG Yun-yang, WU Fang-hui, ZHANG Jing, . Establishment of sandwich immuno-PCR technique for detection of SEB[J]. Chinese Journal of Public Health, 2004, 20(7): 832-833.
Citation: SONG Yun-yang, WU Fang-hui, ZHANG Jing, . Establishment of sandwich immuno-PCR technique for detection of SEB[J]. Chinese Journal of Public Health, 2004, 20(7): 832-833.
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Establishment of sandwich immuno-PCR technique for detection of SEB

    Abstract
  •   Objective   To develop sandwich immune-PCR system for monitoring antigen.
      Methods   Avidin was used to bridge the biotinylated antibodies and biotinylated DNA, and to detect antigen by immuno-PCR.
      Results   As little as 0.1ng/L of SEB could be detected by the sandwich immuno-PCR, which was 103 times more sensitive than ELISA parallel control.
      Conclusion   The universal immuno-PCR system can be used to detect ultra-micro antigen.
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