Effects of Se on COX-2 mRNA expression

  Objective   To investigate effects of Se, in the form of sodium selenite, on COX-2 mRNA expression, a cancer close related gene.

  Methods   The amount of COX-2 mRNA of HT-29 cells, which were cultured in DMEM containing the concentration of 250, 500 and 1 000 nmol/L Se for 4, 8 and 24 h, was detected by real time PCR.

  Results   The expression of COX-2 mRNA were down regulated significantly by 500 and 1 000 nmol/L Se for 24 hours.

  Conclusion   Se had chemoprotective effect on human colon cancer through down regulating COX-2 gene expression and did good to man's suitable diet and offer a background for develoing Se anticancer drug.