Construction and expression of recombinant expression vector of SARS virus E2 protein

  Objective   To construct expression vector and expression in E.coli of SARS virus E2 protein.

  Methods   the E2 gene was inserted into pBV220 vector, the recombinant vector of E2 was identificated by restriction endouclease digestion and PCR.E2 protein was expressed in E.coli via induction in 42℃.

  Results   the analysis for recombinant vector digested by EcoR I and Pst I and PCR demonstrated that the E2 gene was inserted into pBV220.SDS-PAGE showed that the relative molecular mass(Mr)of the expressed E2 protein was about 16600.

  Conclusion   Expression vector of E2 protein was constructed successfully and expressed effectively in E.coli.these results provided an essential preparation for obtaining a larger quantity of recombinant E2 protein for further study.