Detection of vibrio cholerae with DNA microchips

  Objective   Rapid specific and sensitive DNA microchips are used for detection of vibrio cholerae.

  Methods   Primers and specific probes were designed from vibrio cholerae gene ompW and ctxA.The fluorescent-labeled PCR products were prepared by incorporation of Cy3-labeled primer during PCR amplification and were hybridized to the microchips for detection of vibrio cholerae strains and non vibrio cholerae pathogens.

  Results   Positive signal were all displayed on the mycrochips in vibrio cholerae strains when used conventional PCR or multiplex PCR, but non vibrio cholerae pathogens failed to yield any signal under comparable conditions.

  Conclusion   DNA microchips provide a more specific, rapid and efficient method for vibrio cholerae detection.It might be very useful for high-throughout, integrated and automated identification and detection of bacterial pathogens by designing more probes.