Detection for hantavirus in chigger mite with microarray techniques

  Objective   To establish a rapid, sensitive and specific diagnostic assay for Hantavirus with microarray techniques.

  Methods   Specific primers and probes were designed according to the conservative and specific DNA sequence of 76118 strain and R22 strain.The probes were spotted on glass slides to form microarrays.The Cy3-labled single strand DNA fragment prepared by dissymmetical PCR were hybridized with the probers on the glass slides.The microarrays were scanned and analyzed with a scanner.

  Results   DNA microarray could detect the different typed DNA of HTN and SEO with adequate specificity and sensitivity.

  Conclusion   The successfully developed DNA microarray and techniques might be a very useful method for diagnosis and prevention, and widely applied in specific pathogen detection of infectious diseases such as hemorrhagic fever with renal syndrome.