Inhibitory effect of grape procyanidin on abnormal expression of parp mRNA in rat's hepatic cells

  Objective   To study the effect of grape procyanidin(GPC)on N-nitroso compounds-induced expression of poly(ADP-ribose)polymerase(PARP)mRNA in rat's hepatic cells.

  Methods   0.5% NaNO₂ was infused into Wistar rats to induce hepatocytes.mutation, and GPC was infused by 100 and 10 mg/kg respectively through mouth of the two experimental groups at the same time.Aftereight weeks, hepatic tissue was fixed in vivo and situ hybridization was applied to measure the expression level of PARP mRNA in rats.hepatic cells, and its expression level was quantitatively analyzed by an image analysis instrument.

  Results   The positive cells rate of PARP mRNA expressed in the induced-injury group was 30.12% and optical density was 0.354 3±0.041 9, and those of the high-dose GPC group were 11.48% and 0.268 7± 0.017 2, and the difference between the two groups had statistical significance(P < 0.01).

  Conclusion   GPC had inhibitory effect on N-nitroso compounds-induced abnormal expression of PARP mRNA in hepatic cells of rats.