Sequence analysis on hemagglutinin gene of mealses virus isolated in Shenzhen

  Objective   To explore the genetic characterization and genotype of measles viruses circulating in Shenzhen city during the year of 2000-2002.

  Methods   Extracting RNA from throat swabs and urine of the measles patients, using RT-PCR amplification measles virus hemagglutinin gene of 571bp.PCR products were cloned into PM D18-T vector and the homogeneity of the virus isolated were compared with the strains in genebank.

  Results   H genes homogeneity was between the range of 96.2%-100% compared with other wild-type viruses of H₁ genoty pe, the homogeneity was 96.4%-99.3%.

  Conclusion   Shenzhen circulating measles viruses were belong ed to the clade H₁.