Effects of vitamin on nitric oxide synthase activity of alveolar macrophage

  Objective   In order to study the effects of vitamin E and vitamin C on the injury of alveolar macrophages(AM) exposed to quartz in vitro.

  Methods   Different dosages of vitamin E and vitamin C were respectively added into AM medium, cultured with exposure of quartz in vitro.Cell activities of the three groups were observed.Also nitric oxide(NO) and the activity of nitric oxide synthase(NOS) in AM were carried out.

  Results   Vitamin E and vitamin C could increase survival rate and the activity of AM.At the same time, activities of NO and NOS were decreased, especially the combination of VE and VC both at 100 μmol/L consistency, having the dramatic inhibitory effect.

  Conclusion   VE and VC can inhibit the activity of NOS, decrease the amount of NO, antagonize the injury of AM exposed to quartz.