Investigation on factors affecting purification of HAV with chloroform

  Objective   To investigate the factors relating to the purification of HAV.

  Methods   It was performed by the orthogonal experiment method to analyze some factors relating to the purification of HAV with chloroform.

  Results   The matches between the powers of sonication and the sample volumes of virus were important factors in the titration of virus.It was better to use PBS and NS as washing buffer than MEM.The quality of chloroform also influenced the purification of HAV.

  Conclusion   The purification of HAV was influenced by several factors, and it was necessary to optimize these factors to reduce the influence on the activity of HAV.