Effects of chlorfenapyr on DNA damage of spleen cells and liver cells in mice by single cell gel electrophoresis

  Objective   To study the effects of Chlorfenapyr on DNA damage of spleen and liver cells in mice.

  Methods   Chlorfenapyr was administrated to mice with 4.9, 9.8 and 19.6 mg/kg doses once by gavage, then the percentage of cells with comets and the DNA migration of spleen cells and liver cells were detected by single cell gel electrophoresis.

  Results   Compared with the control group, the percentage of cells with comets and the DNA migration were increased significantly at all the three tested doses of the two kinds of tissue cells(P < 0.05-0.001);except for the DNA migration of spleen cells, the percentage of cells with comets of spleen cells and liver cells and the DNA migration of liver cells exhibited a significant linear dose-dependent increase(r values were 0.995, 0.9987 and 0.9999 respectively, P < 0.05);at the identical dosage criterion, the percentage of cells with comets of liver cells increased statistically at all the three tested doses as compared to the corresponding dose spleen cells groups(P < 0.01), so did the DNA migration of the 4.9 mg/kg dose of liver cells(P < 0.05).

  Conclusion   Chlorfenapyr can induce the DNA damage of spleen cells and liver cells, and the effects on the DNA damage of liver cells is stronger than that of spleen cells.