Study on oxidative damage effects of cooking oil fume conden sation on BEAS-2B cell

  Objective   The reactive oxygen species(ROS)production and A TPase in human bronchial epithelial cell line induced by cooking oil fume condensation were studied through measuring extra celluar and intracellular ROS levels including super oxide anions(₂)and hydrogen peroxides(H₂O₂), and ATPase contents.

  Methods   Sepectrometric method and fluorometric method were used to measure the ROS reduced by COFC.And the activities of ATPase assayed by biochemical method.

  Results   The ROS correlated with the decrease of contents of A TPase.

  Conclusion   Membrane ATPase activity was inhibited by ROS, especially Ca²⁺ATPase and Mg²⁺ATPase.