Rapid and sensitive screening method for detecting estrogenic compounds in environmental hormone

  Objective   To investigate a rapid and sensitive scr eening method for detecting estrogenic compounds in enviromnental hormone.

  Methods   Both expression plasmid pcDNA31.1-ER and repor ter plasmid pGL3-tk-ERE were constructed by inserting cDNA of human estrogen receptor-α(ER-α)into plasmid pcDNA31.1 and estrogen receptor response element(ERE)into reporter plasmid pGL3-tk, respectively.After cotransfection the two plasmids into Hela cells, the luciferase activity induced by 17-β-estradiol(E₂)or Bisphenol A(BPA)through the interaction of ER with ERE were detected.

  Results   the activity of enzyme regulated by ERE in the presence of E₂ and BPA were linearly correlated with the concentration of E₂ and BPA within a certain dose range.

  Conclusion   this method was suitable for detection of estrogen and estrogenic compounds.With further development, it would become a rapid and efficient routine method for detection of estrogenic compounds in the environmental hormone.