DNA damage and repair of human lymphocytes treated with cigarette smoke solution

  Objective   To learn DNA damage and its repair in normal human lymphocytes treated with cigarette smoke solution(CSS).

  Methods   Human lymphocytes were exposed to 8×10^-3 cig/ml CSS for0, 0.5, 1, 2, 3, 4, 5and6 hrespectively, then DNA damage was evaluated by comet assay.

  Results   T ail leng ths of comet in groups exposed to CSS from 2 to 6 h were significantly longer than that of the control(0 h)(P < 0.01).It reached the longest at 3 h and decreased subse-quently.However, it did not reduce to normal level at 6 h.

  Conclusion   CSS could induce DNA damage of human lymphocytes, and the damage could be repaired to some extent.