Effects of domoic acid on heme oxygenase-1 protein expression in mouse brain

  Objective   To study the effects of domic acid on MDA content and heme oxygenase-1(HO-1)protein expression in mouse brain.

  Methods   TBA method and immunity histochemistry staining were applied to assess the MDA content and HO-1 protein expression respectively in mouse brain after administration domoic acid, ip for 5 days.

  Results   the MDA content in hippocampus in low dose group(1/90 LD₅₀)and in high dose group(1/10 LD₅₀)were significantly different from that in hippocampus in control group(P < 0.05).The MDA content in cortex of high dose group was significantly different form that in cortex in control group(P < 0.05).The mean light activity of positive HO-1 cells respectively was 0.141 0±0.040 7 in hippocampus and 0.140 6±0.035 4 in cortex in low dose group, respectively higher than 0.095 3±0.025 2 in hippocampus and 0.094 0±0.030 4 in cortex in control group(P < 0.05).The mean light activity of positive HO-1 cells respectively was 01162 6±0.037 4 in hippocampus and 0.160 4±0.032 5 in cortex in high dose group, respectively higher than that in hippocampus and in cortex in control group(P < 0.01).

  Conclusion   Domoic acid could cause oxidative injury in hippocampus and cortex in mouse brain and increase HO-1 protein expression as well.