Effects of formaldehyde on MGMT in mice organs

  Objective   To study the effects of formaldehye(FA)on expression of MGMT in mice organs.

  Methods   40 healthy Kunming mice were selected and randomly divided into 4 groups, 10 mice per group, FA low-dose group(2.5 mg/m³), FA middle-dose group(5 mg/m³), FA hig h-dose group(10 mg/m³), experimental control group(exposed no FA).Except the control group, the other 3 groups were exposed to FA by inspiration in a static total enclosure chamber 4 hours once a day for 9 consecutive weeks.After exposure, the MGMT in brain, lung, liver and kidney was examined by SABC immuno histo chemical staining.

  Results   the expression of MGMT in liver and lung in all experimental groups were downregulated compared with the control(P < 0.05 orP < 0.01).MGMT expression of brain and kidney did not change after exposure to FA.

  Conclusion   FA could decrease the expr ession of MGMT in liver and lung in mice.