Fas signaling transduction pathway of vitamin E succinate-induced apoptosis in human gastric adenocarcinoma cell

  Objective   To study the fas signaling transduction pathway of vitamin E succinate(VES)-induced apoptosis in human gastric adenocarcinoma cells.

  Methods   Human gastric adeno carcinoma cells were treated with VES at 5, 10, 20 μg/ml, succinic acid, vitamin E and untreated control.Apoptotic morphology was observed by DAPI staining.Western Blot analysis was applied to measure the expression of fas, FADD and caspase-8 proteins.After the cells were transiently transfected with fas and FADD antisense oligonucleotides, respectively, caspase-8 activity was determined by flurometric method.

  Results   The morphologically apoptotic changes were observed after VES treatment by DAPI staining.89.6% apoptosis occurred after 48h of 20 μg/ml VES treatment.The protein levels of fas, FADD and caspase-8 were evidently increased in a dose-dependent manner after 48h of VES treatment.The blockage of fas by transfection with fas antisense oligonucleotides obviously inhibited the expression of FADD protein.After SGC-7901 cells were transfected with fas and FADD antisense oligonucleotides, caspase-8 activity was obviously decreased(P < 0.01), whereas fas blocked more than FADD.

  Conclusion   VES-induced apoptosis in human gastric adenocarcinoma SGC-7901 cells involved fas signaling transduction pathway including the interaction of fas, FADD and caspase-8.That was fas/FA DD/caspase-8 signaling transduction pathway.