Objective To study the effects of c9, t11-conjugated linoleic acid on the killing ability of macrophage to B16-MB cells in C57 mice and explore its possible mechanism.
Methods Five levels of CLA was designed as 0, 25, 50, 75, 100 μmol/L.After macro phage was treated with CLA for 24 h, the MTT assay was used to evaluate the killing ability of macrophage to B16-MB cells; the RT-PCR was used to detect the expression of macrophage cytokine IL-6, T NF-a and iNOS mRNA in C57 mice.
Results At the concentrations of 100, 75 μmol/L c9, t11-CL A, the cytotoxic effects of macrophage on tumor cells were 18% and 14.5%, respectiely.At the same time, the expression of IL-6, TNF-A and iNOS mRNA increased.
Conclusion c9, t11-CLA could increase the killing ability of macrophage to B16-MB cells in mice, and was associated with the induction of IL-6, T NF-A and iNOS mRNA expression.Antitumor ability of CLA may be asso ciated with taking part in immune regulation action.
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