Objective To identify the HIV-1 subtype epidemic in Liaoning by nucleotide sequencing.
Methods Eight of the selected 16 HIV-1 sero-positive individuals were infected with HIV-1 by sexual contacts, 7 were IDUs or blood recipients and blood donors, 1 was vertical transmitant.DNA frag ments of HIV-1 envgene were amplified by PCR from provirus in human genome.The amplified products of C2-V3 region of HIV-1 were sequenced and compared with the consensus sequence of reference HIV-1 strains and some strains epidemic in Aisa.
Results HIV-1 strains epidemic in Liaoning were found to be of genetic subtype A, B., C, G.8 of sexual transmission were subtype B.and A.4 of IDUs were subtype C.Blood recipients and blood donors were of subtype B.and G, 1 of vertical transmit ant was subtype A.
Conclusion There was several subtypes HIV-1 invading Liaoning province, which manifested the complexity of HIV epidemiology in Liaoning province.The HIV-1 subtype in different transmission route showed respective character: Subtype C were found only in IDUs, individuals infected HIV-1 by sexual contact and blood route were mainly of subtype B., however subtype A and G can only be found in laborer returned from Africa and his spouse and children.