Objective To study the effect of flavonoid of celosia cristata extract on mineralization and IGF-1 expression of cultured osteoblasts of rat.
Methods The osteoblastic cells from cranlial bone of newborn rat was cultured.The effect of mineralization was detected by alizarin bordeaux stain(ARS).The effect of flavonoid of celosia cr istata extract on IGF-1 expression of cultured osteoblasb was detected by SP method.The expression level was monitored with HPIAS-1000 photograph analysismeter.
Results The IGF-1 of third cultured osteoblasts cultured used flavonoid of celosia cristata extract was positive expression.The IGF-1 positive expression of flavonoid of celosia cristata extract group was higher significantly than negative control group(P < 0.05).
Conclusion The flavonoid of celosia cristata extract may increase the mineralization and IGF-1 positive expression of cultured osteoblasts of rat.