Lipid peroxidation injuries of hepatic subcellular fractions in mice caused by chronic fluorsis, arsenism poisoning

  Objective   To study the levels, similarities and differences of the lipid peroxidation injury effects in hepatic subcellular fractions caused by chronic fluorosis, arsenism and fluoride-arsenic poisoning respectively in mice.

  Methods   120 mice were randomly divided into four groups which were fed with four different diets supplemented respectively fluoride arsenic and fluoride-arsenic for 6 and 12 months.The LPO contents in hepatic mitochondria and microsomes and the activities of SDHase in hepatic mitochondria and G-6-Pase in hepatic microsomes were determined.

  Results   In the three poisoning groups: the LPO contents in hepatic mitochondria and microsomes were significally increased.The activities of SDHase in hepatic mitochondria and G-6-Pase in hepatic microsomes were markedly decreased.The increases of LPO contents in two subcellular fractions showed significally negative correlation to the decreases of their enzymes activities, respectively.However, the effect of the three diffierent poisons on these indices were different.

  Conclusion   In mice, the lipid peroxidation injuries in hepatic mitochondria and microsomes were caused by the chronic three poisons, to some extent, fluoride and arsenic can influence the effect together.